High production of wheat double haploids via anther culture

Anther culture of iranian wheat germplasm using different media

Colchicine induced embryogenesis and doubled haploid production in maize (Zea mays L.) anther culture

This study involves in vitro androgenesis of <sp...

Genetic Transformation of Wheat (triticum Aestivum L.) Anther Culture- Derived Embryos by Electroporation

This report describes the delivery of plasmid DNA into wheat anther culture derived embryos. Initial electroporation experiments were conducted using enzyme (NT-1) pretreatment, 500 F capacitance, and plasmid pAM2100, which carries the herbicide resistance gene, bar, and the screenable marker gene, Gus. Electroporated embryos were allowed to recover 10 days on regeneration medium and then trans...

Doubled Haploids versus Conventional Breeding in CIMMYT Wheat Breeding Programs

Doubled haploid (DH) technology has been used in breeding programs for several decades and is currently the method of choice in a number of crop species, including barley (Hordeum vulgare L.), rapeseed (Brassica napus L.), maize (Zea mays L.), and wheat (Triticum aestivum L.). In this study we investigated via computer simulation the benefit of using DHs compared with the conventional wheat bre...

مقایسه روش‌های تلاقی گندم * ذرت و کشت بساک در تولید گیاهان هاپلویید گندم

The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization a...